1: J Urol. 2009 Jun;181(6):2806-15. Epub 2009 Apr 17.
Vcsa1 acts as a marker of erectile function recovery after gene therapeutic and pharmacological interventions.
Calenda, Giulia; Tong, Yuehong; Tar, Moses; Lowe, Daniel; Siragusa, Joseph; Melman, Arnold; Davies, Kelvin P;
Department of Urology and Institute of Smooth Muscle Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
PURPOSE: We identified molecular markers of erectile function, particularly those responding to erectile dysfunction treatment. MATERIALS AND METHODS: Sprague-Dawley retired breeder rats were intracorporeally injected with pVAX-hSlo, pSMAA-hSlo or the control plasmid pVAX. One week later the intracorporeal pressure-to-blood pressure ratio and gene expression were determined by microarray analysis and quantitative reverse transcriptase-polymerase chain reaction. Rat corporeal cells were transfected in vitro with pVAX-hSlo, pSMAA-hSlo or pVAX and the change in gene expression was determined. We also determined whether Vcsa1 expression was changed after pharmacotherapy using tadalafil. RESULTS: Animals treated with vectors expressing hSlo had significantly improved erectile function compared to that in controls, accompanied by changed expression of a subset of genes. Vcsa1 was one of the genes that was most changed in expression (the third of approximately 31,000 with greater than 10-fold up-regulation). Changes in gene expression were different than those observed in corporeal cells transfected in vitro, distinguishing gene expression changes that were a direct effect of hSlo over expression. When tadalafil was administered in retired breeder rats, the Vcsa1 transcript increased 4-fold in corporeal tissue compared to that in untreated controls. CONCLUSIONS: Our study identifies a set of genes that are changed in response to improved erectile function, rather than as a direct effect of treatment. We noted Vcsa1 may act as marker of the restoration of erectile function after gene transfer and pharmacotherapy.
  • Masculino
  • Animales
  • Ratas
  • Ratas Sprague-Dawley
  • Expresión Génica
  • Terapia Génica
  • Inhibidores de la Fosfodiesterasa (uso terapéutico)
  • Erección Peniana (fisiología)
  • Carbolinas (uso terapéutico)
  • Precursores de Proteínas (genética, fisiología)
  • Salivary Proteins and Peptides (genética, fisiología)
  • Disfunción Eréctil (quimioterapia, genética, terapia)

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